With the rapid advancement of the biopharmaceutical field, peptide-based biologics have demonstrated clear advantages over small-molecule therapeutics in disease treatment, such as high pharmacological activity, high solubility, and low toxicity. However, conventional peptide drug formulations still face a range of clinical and logistical challenges:

Preliminary in vivo biocompatibility in mice was satisfactory, with no skin irritation and normal complete blood count, renal, and hepatic function. In vivo studies in a rabbit ear HS model demonstrate accelerated HS healing, reduced HS thickness, epidermal thickness index (ETI), scar elevation index (SEI), and collagen deposition, along with decreased profibrotic markers, inflammatory cytokines, and angiogenic factors. Overall, the TS+GLNPs@sMN patch offers a minimally invasive, self-administrable, dual-action therapeutic strategy for enhanced HS management, with safety and efficacy in preclinical models.

This study comprehensively analyzes the top 100 highly cited publications in microneedle (MN) research to reveal convergences and divergences in academic and public interest, assess the current research landscape and its impact, and offer data-driven support for clarifying future research directions.We identified highly cited microneedle articles published from 2015 to July 15, 2025 using the Web of Science database, validated the records through Scopus and Google Scholar, and gathered Altmetric attention scores from Altmetric.Extracted data encompassed citation counts, publication years, journals, authors, affiliations, and countries, which we summarized using descriptive statistics and visualized with CiteSpace, VOSviewer, and R for correlation analysis.

The present study demonstrated the successful design and development of dissolving microneedle patches with an advanced architecture for transdermal delivery of naltrexone hydrochloride. The combination of PVP/PVA-based microneedle tips and a PVP/Poloxamer 407 backing layer proved to be an effective strategy for balancing mechanical performance and drug delivery characteristics. The selected polymer blend for the tips ensured adequate mechanical strength for insertion while maintaining suitable dissolution behavior, whereas the thermoresponsive backing layer functioned as a drug reservoir capable of modulating release and permeation.

Smart hydrogel dressings represent a significant advancement in treating complex wounds such as severe burns and chronic diabetic ulcers, which conventional materials struggle to address effectively. These innovative hydrogels closely mimic the native extracellular matrix while offering tunable physicochemical properties and high water content. Recent developments encompass natural and synthetic polymers alongside bio-inspired systems including DNA-based and peptide hydrogels. Key multifunctional capabilities include stimulus-responsive drug delivery, antimicrobial and antioxidant properties, immunomodulatory effects, and enhanced mechanical characteristics such as self-healing and tissue adhesion. By targeting specific pathological mechanisms including macrophage polarization, angiogenesis, cellular senescence, and fibrosis, these advanced hydrogels facilitate comprehensive tissue regeneration beyond simple wound closure, offering promising therapeutic potential for complex wound management.

Evaluate pH level and temperature stability of HA to achieve optimal virus uptake. High-density microarray patch (HD-MAP) delivery of a DNA vaccine evaluate ...

At lower temperatures, the polymer chains are hydrated and exist as unimers, while increasing temperature enables dehydration of the polypropylene oxide blocks, ...

Researchers have developed an innovative wearable paper-based microneedle array sensor for continuous glucose monitoring that addresses current limitations in diabetes management. The system integrates disposable solid microneedles fabricated from biocompatible surgical guide resin and microcrystalline cellulose on a flexible paper substrate, paired with reusable electronics and a mobile application. The microneedles function as electrochemical biosensors through graphene coating and functional modifiers, enabling real-time glucose detection in dermal interstitial fluid. Extensive testing on porcine plasma, artificial skin models, and live mice demonstrates high sensitivity, selectivity, and stability. This cost-effective, eco-friendly approach represents a significant advancement toward practical continuous glucose monitoring, potentially improving patient adherence and acceptance in diabetes care.

Diabetic, chronic, and refractory wounds formed a central application area because they involve overlapping barriers to repair, including persistent ...

Chitosan-based wound dressings represent a promising therapeutic approach for managing chronic wounds, which present multiple healing challenges including infection, vascular insufficiency, and immune dysregulation. Derived from chitin deacetylation, chitosan is a biocompatible and biodegradable polysaccharide with inherent hemostatic and antibacterial properties that can be chemically modified to enhance efficacy. This comprehensive review examines how structural modifications and formulation strategies, such as catechol grafting and quaternization, enable chitosan-based dressings to address specific pathological barriers in different wound types. By systematically linking wound pathophysiology to functional dressing requirements, the research demonstrates that therapeutic effectiveness depends not solely on chitosan's intrinsic properties but rather on targeted property modulation through rational design and fabrication approaches.

Transdermal patches represent an innovative approach to wound healing through controlled drug delivery. These patches are classified into four main types, with drug-in-adhesive and matrix-type patches proving most effective. Drug-in-adhesive patches disperse medication uniformly within an adhesive layer, offering simplicity and flexibility for sustained wound contact. Matrix-type patches utilize polymeric materials such as chitosan, polyvinyl alcohol, and polyurethane to release therapeutic agents gradually while maintaining optimal moisture levels and protecting wounds from contamination. Reservoir-type patches provide precise drug control through liquid or gel reservoirs between membranes. This technology safely delivers antimicrobial agents, anti-inflammatory mediators, and growth factors, making transdermal patches a valuable therapeutic option for enhanced wound healing outcomes.

# Professional Summary Researchers have developed an innovative approach to accelerate diabetic wound healing through the integration of nanoenzyme-engineered hydrogels with microneedle technology and visible light therapy. The ACMGM microneedle system, when combined with phototherapy, demonstrates enhanced therapeutic efficacy by reprogramming the wound microenvironment. This breakthrough addresses a critical clinical challenge, as diabetic patients experience impaired wound healing due to compromised metabolic and immune responses. The nanoenzyme-based hydrogels work synergistically with light stimulation to promote cellular regeneration and tissue repair. This multifaceted therapeutic strategy represents a significant advancement in regenerative medicine and offers promising potential for improving clinical outcomes in diabetic wound management.

Researchers have developed an innovative closed-loop delivery system that combines nitric oxide and hydrogen with feedback control mechanisms to enhance diabetic wound healing. This advanced therapeutic approach leverages synergistic effects between the two gaseous compounds, optimizing their release based on real-time wound conditions. Published in Nano-Micro Letters, this open-access research represents a significant advancement in biomedical engineering and regenerative medicine. The closed-loop feedback system enables precise, responsive treatment that adapts to individual healing progression, potentially improving outcomes for patients with diabetes-related complications. This breakthrough offers promising applications for addressing the substantial clinical challenge of impaired wound healing in diabetic populations.

Continuous glucose monitoring through wearable technology addresses critical needs for diabetes management, yet current microneedle approaches face practical limitations. This research presents an innovative disposable microneedle array integrated with flexible paper substrate and reusable electronics for electrochemical glucose sensing in dermal interstitial fluid. The solid microneeles are fabricated from a biocompatible composite of surgical guide resin and microcrystalline cellulose, coated with graphene ink for conductivity. Extensive testing on porcine plasma, artificial skin, and live mice demonstrated high sensitivity, selectivity, and stability. This cost-effective, eco-friendly solution offers significant advancement toward practical, patient-friendly continuous glucose monitoring devices with enhanced wearability and compliance.

Traditional Chinese medicine (TCM) has abundant medicinal resources and distinctive pharmacological properties. So, TCM presents considerable promise in clinical treatments. However, challenges such as poor bioavailability hinder broader clinical adoption of TCM. Microneedles (MNs), an innovative and minimally invasive transdermal platform, have emerged to enhance the therapeutic performance of TCM. The integration of MNs with TCM (TCM-MNs) overcomes key limitations of conventional administration routes to reach more targeted and efficient delivery. The structural and compositional diversity of TCM ingredients necessitates diverse TCM-MNs designs, especially "unification of medicines and excipients".

The purpose of this research study is to check whether the MNS Miracus Patch (the study's investigational device, a small patch made up of microneedles that dissolve in the skin and can potentially deliver vaccines in a pain-free way) is safe to use and tolerated by humans when administered for 10 minutes, both as a plain patch (with no vaccine) and as a loaded patch (with the tetanus- containing vaccine included). This study will also investigate whether the patch delivers the correct dose of the vaccine in the loaded patches by checking the immune response of participants.As the device has not been tested clinically in humans before, this will be a first-in-human study involving healthy participants only, who have not previously received the full course of tetanus diphtheria vaccinations. This research study will take approximately 4-6 weeks of the participants' time.

Peri-implantitis management is undergoing a significant paradigm shift from conventional mechanical debridement toward immunopharmacological approaches. This comprehensive review examines the molecular mechanisms underlying peri-implantitis, highlighting its complex immunological nature through single-cell sequencing studies and cell-interaction patterns. The research synthesizes evidence on host-modulatory therapies, including targeted anti-cytokine biologics and specialized pro-resolving mediators, alongside advanced drug delivery systems such as stimuli-responsive hydrogels and exosome-based platforms. While these innovative therapeutic strategies demonstrate considerable promise in preclinical and early translational stages, most require prospective clinical validation. Additionally, machine-learning models emerge as valuable tools for risk stratification. This emerging evidence-based framework offers substantial potential for improving peri-implantitis treatment outcomes through precision immunopharmacology.

Three-dimensional printing technologies are transforming biosensing by enabling the creation of fully integrated devices that consolidate sample preparation, fluid handling, sensing elements, and structural components within a single manufacturing process. This approach significantly minimizes manual assembly requirements, reduces sample volumes, and enables rapid analysis suitable for point-of-care or wearable applications. The perspective examines recent advances in 3D-printed biosensors across biomedical, environmental, and wearable domains, evaluating various additive manufacturing techniques including fused deposition modeling, stereolithography, and digital light processing. The analysis emphasizes the critical relationship between material properties and functionality, addressing filament preparation, substrate selection, and surface functionalization strategies. The article identifies key technical challenges such as resolution limitations, material conductivity, biomolecule stability, and sustainability concerns while proposing solutions through hybrid printing approaches and innovative low-temperature methods.

University of Connecticut researchers have developed a microneedle patch vaccine that addresses significant challenges in foot and mouth disease prevention for livestock. This innovative delivery system eliminates the need for traditional needle injections, which are often the most difficult aspect of vaccine distribution on farms. Foot and mouth disease causes severe economic damage, with potential losses reaching $228 billion from major US outbreaks. The existing adenovirus-based vaccine requires cold-chain maintenance and careful preparation, creating logistical complications and contamination risks. Additionally, injection marks reduce meat quality and market value. The temperature-stabilized patch vaccine offers a safer, more practical alternative that simplifies administration while maintaining vaccine efficacy, potentially transforming disease prevention in cattle, sheep, and goat populations.

Researchers have developed innovative microneedles with light- and temperature-dependent properties to enhance transdermal drug delivery. While traditional transdermal administration offers significant advantages such as avoiding first-pass hepatic metabolism and improving patient compliance, the skin's barrier function limits drug permeation. This advancement addresses a critical challenge in the field by enabling controlled, responsive drug release through the skin. The new microneedles leverage photochemical and thermal stimuli to optimize penetration while maintaining structural integrity, potentially overcoming mechanical limitations of conventional microneedle matrices and advancing clinical applications in personalized medicine.